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Small angle X-ray and neutron scattering from solutions of biological macromolecules

Author: D I Svergun; Michel H J Koch; Peter A Timmins; Roland P May
Publisher: Oxford ; New York : Oxford University Press, 2013.
Series: International Union of Crystallography texts on crystallography, 19.; Oxford science publications.
Edition/Format:   Book : English : First editionView all editions and formats
Database:WorldCat
Summary:
In this book, following the presentation of the basics of scattering from isotropic macromolecular solutions, modern instrumentation, experimental practice, and advanced analysis techniques are explained. Advantages of X-rays (rapid data collection, small sample volumes) and of neutrons (contrast variation by hydrogen/deuterium exchange) are specifically highlighted. Examples of applications of the technique to  Read more...
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Details

Document Type: Book
All Authors / Contributors: D I Svergun; Michel H J Koch; Peter A Timmins; Roland P May
ISBN: 9780199639533 0199639531
OCLC Number: 858076801
Description: ix, 358 pages : illustrations ; 26 cm.
Contents: Machine generated contents note: pt. I THEORY AND EXPERIMENT --
1.Basics of small angle scattering --
1.1.Elastic scattering of X-rays and neutrons --
1.2.Scattering by macromolecular solutions --
1.3.Resolution and contrast --
1.4.Absorption and anomalous X-ray scattering --
References --
2.X-ray and neutron scattering instruments --
2.1.Characteristics of sources --
2.2.X-ray sources --
2.3.Neutron sources --
2.4.SAXS instruments --
2.5.SANS instruments --
2.6.Special instruments --
References --
3.Experimental practice and data processing --
3.1.Sample requirements for SAXS and SANS --
3.2.Experimental protocols and instrumental corrections --
3.3.Special features of SAXS: radiation damage --
3.4.Special features of SANS: the use of H2O/D2O mixtures --
3.5.Random and systematic errors --
3.6.Basic structural information and data quality --
3.7.Calibration to absolute scale and molecular mass --
References --
pt. II DATA ANALYSIS METHODS --
4.Monodisperse systems --
Contents note continued: 4.1.Overall parameters of particles --
4.2.Multipole representation of SAS intensity --
4.3.Shannon sampling --
4.4.Ab initio shape analysis --
4.5.Computation of scattering patterns from atomic models --
4.6.Hybrid methods --
4.7.Labelling and triangulation --
References --
5.Polydisperse and interacting systems --
5.1.Size, shape and conformation polydispersity --
5.2.Size distribution functions --
5.3.Shape polydispersity and oligomeric mixtures --
5.4.Conformational polydispersity and flexible systems --
5.5.Interacting systems and structure factor --
References --
pt. III BIOLOGICAL APPLICATIONS OF SOLUTION SAS --
6.Static structural studies --
6.1.Applications of ab initio shape determination --
6.2.Quaternary structure analysis of proteins and complexes --
6.3.Equilibrium mixtures and oligomeric composition --
6.4.Membrane proteins and lipoproteins --
6.5.Flexible systems --
References --
7.Kinetic and perturbation studies --
Contents note continued: 7.1.Dynamics and kinetics --
7.2.Perturbation methods --
7.3.Temperature scans and T-jumps --
7.4.High-pressure experiments --
7.5.Stopped-flow and continuous-flow mixing --
7.6.Light-triggered processes --
References --
8.Analysis of interparticle interactions --
8.1.Basic physical chemistry of interactions --
8.2.Experimental SAS studies of protein-protein interactions --
8.3.Structure factor calculations for proteins --
8.4.Interactions in nucleic acids --
References --
9.SAS in multidisciplinary studies --
9.1.Automation and high-throughput SAS --
9.2.Joint use with high-resolution methods --
9.3.SAS and low-resolution crystallography --
9.4.Complementary biophysical methods --
9.5.Bioinformatics and model validation --
References --
Conclusions and future prospects --
Appendices --
Appendix 1 Basic physics and mathematics of wave phenomena --
Appendix 2 Spherical harmonics and their applications for SAS --
Contents note continued: Appendix 3 Interactions between spherical molecules --
Appendix 4 Web resources.
Series Title: International Union of Crystallography texts on crystallography, 19.; Oxford science publications.
Responsibility: Dmitri I. Svergun, Michel H.J. Koch, Peter A. Timmins, Roland P. May.

Abstract:

This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a  Read more...
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This book is a masterly treatment by four authors with many decades of experience of SAXS and SANS and who are from world class synchrotron X-ray and neutron research facilities. From the basics and Read more...

 
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