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Small angle X-ray and neutron scattering from solutions of biological macromolecules

Author: D I Svergun; Michel H J Koch; Peter A Timmins; Roland P May
Publisher: Oxford ; New York : Oxford University Press, 2013.
Series: International Union of Crystallography texts on crystallography, 19.; Oxford science publications.
Edition/Format:   Book : English : First editionView all editions and formats
In this book, following the presentation of the basics of scattering from isotropic macromolecular solutions, modern instrumentation, experimental practice, and advanced analysis techniques are explained. Advantages of X-rays (rapid data collection, small sample volumes) and of neutrons (contrast variation by hydrogen/deuterium exchange) are specifically highlighted. Examples of applications of the technique to  Read more...
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Document Type: Book
All Authors / Contributors: D I Svergun; Michel H J Koch; Peter A Timmins; Roland P May
ISBN: 9780199639533 0199639531
OCLC Number: 858076801
Description: ix, 358 pages : illustrations ; 26 cm.
Contents: Machine generated contents note: pt. I THEORY AND EXPERIMENT --
1.Basics of small angle scattering --
1.1.Elastic scattering of X-rays and neutrons --
1.2.Scattering by macromolecular solutions --
1.3.Resolution and contrast --
1.4.Absorption and anomalous X-ray scattering --
References --
2.X-ray and neutron scattering instruments --
2.1.Characteristics of sources --
2.2.X-ray sources --
2.3.Neutron sources --
2.4.SAXS instruments --
2.5.SANS instruments --
2.6.Special instruments --
References --
3.Experimental practice and data processing --
3.1.Sample requirements for SAXS and SANS --
3.2.Experimental protocols and instrumental corrections --
3.3.Special features of SAXS: radiation damage --
3.4.Special features of SANS: the use of H2O/D2O mixtures --
3.5.Random and systematic errors --
3.6.Basic structural information and data quality --
3.7.Calibration to absolute scale and molecular mass --
References --
4.Monodisperse systems --
Contents note continued: 4.1.Overall parameters of particles --
4.2.Multipole representation of SAS intensity --
4.3.Shannon sampling --
4.4.Ab initio shape analysis --
4.5.Computation of scattering patterns from atomic models --
4.6.Hybrid methods --
4.7.Labelling and triangulation --
References --
5.Polydisperse and interacting systems --
5.1.Size, shape and conformation polydispersity --
5.2.Size distribution functions --
5.3.Shape polydispersity and oligomeric mixtures --
5.4.Conformational polydispersity and flexible systems --
5.5.Interacting systems and structure factor --
References --
6.Static structural studies --
6.1.Applications of ab initio shape determination --
6.2.Quaternary structure analysis of proteins and complexes --
6.3.Equilibrium mixtures and oligomeric composition --
6.4.Membrane proteins and lipoproteins --
6.5.Flexible systems --
References --
7.Kinetic and perturbation studies --
Contents note continued: 7.1.Dynamics and kinetics --
7.2.Perturbation methods --
7.3.Temperature scans and T-jumps --
7.4.High-pressure experiments --
7.5.Stopped-flow and continuous-flow mixing --
7.6.Light-triggered processes --
References --
8.Analysis of interparticle interactions --
8.1.Basic physical chemistry of interactions --
8.2.Experimental SAS studies of protein-protein interactions --
8.3.Structure factor calculations for proteins --
8.4.Interactions in nucleic acids --
References --
9.SAS in multidisciplinary studies --
9.1.Automation and high-throughput SAS --
9.2.Joint use with high-resolution methods --
9.3.SAS and low-resolution crystallography --
9.4.Complementary biophysical methods --
9.5.Bioinformatics and model validation --
References --
Conclusions and future prospects --
Appendices --
Appendix 1 Basic physics and mathematics of wave phenomena --
Appendix 2 Spherical harmonics and their applications for SAS --
Contents note continued: Appendix 3 Interactions between spherical molecules --
Appendix 4 Web resources.
Series Title: International Union of Crystallography texts on crystallography, 19.; Oxford science publications.
Responsibility: Dmitri I. Svergun, Michel H.J. Koch, Peter A. Timmins, Roland P. May.


This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a  Read more...
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This book is a masterly treatment by four authors with many decades of experience of SAXS and SANS and who are from world class synchrotron X-ray and neutron research facilities. From the basics and Read more...

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